Department of Chemical and Biomolecular Engineering
YAP, Gek Sim Miranda
Professor
PhD (Chem. Eng.) Toronto, 1979
MSc (Chem. Biochem. Eng.) London, 1973
BSc (Appl. Chem.) Singapore, 1972Contact information
20 Biopolis Way #06-01 Centros Singapore 138668
Tel: (65) 6478 8888 Fax: (65) 6478 9561
Email: cheyapm@nus.edu.sg (NUS) miranda_yap@bti.a-star.edu.sg (BTI)Executive Director, Bioprocessing Technology Institute (BTI)
Associate, Molecular Engineering of Biological and Chemical Systems (MEBCS), Singapore-MIT Alliance
SMA Fellow, SMA Program Co-Chair
RESEARCH
Animal Cell Culture
Research focuses on process development, modelling and optimisation of biological systems. Currently we are involved in developing chemically defined media (CDF) for cultivating of Chinese Hamster Ovary (CHO), hybridoma and Human Embryonic Kidney-293 which are industrially important production cell lines. Using CDM will enable us to undertake stoichiometric and metabolic flux analyses and to develop control strategies for fed-batch systems leading to improved metabolism and hence enhance overall productivity. Effects of culture conditions on gene and protein expression profiles are tracked using proteomics (2DE, MALDI and Tandem MS) and DNA microarrays. We are also interested in prolonged fed-batch on protein glycosylation specifically in elucidating cellular responses in an apoptotic environment.
SELECTED PUBLICATIONS
B. T. Koh, R. B. H. Tan and M. G. S. Yap, "Genetically Structured Mathematical Model of Trp Attenuator Mechanism." Biotechnology and Bioengineering, 58, 502 (1998).
F. K. F. Chua, M. G. S. Yap and S. K. W. Oh, "Hyper-stimulation of Monoclonal Antibody Production in Several Cell Lines by High Osmolarity Stress in eRDF Medium", Journal of Biotechnology, 37, 265 (1994).
S. K. W. Oh, P. Vig, F. Chua, W. K. Teo and M. G. S. Yap, "Substantial Overproduction of Antibodies by Applying Osmotic Pressure and Sodium Butyrate", Biotechnology and Bioengineering, 42, 601 (1993).
H. Chai, S. G. Vasudevan, A. G. Porter, K. L. Chua, S. K. W. Oh and M. G. S. Yap, "Glycosylation and High-Level Secretion of Human Tumor Necrosis Factor-b in Recombinant Baculovirus-infected Inset Cells", Biotechnology and Applied Biochemistry, 18, 259 (1993).
B. T. Koh and M. G. S., Yap, "A Simple Genetically Structured Model of trpRepressor-Operator Interaction", Biotechnology and Bioengineering, 41, 45 (1991).
